Ben Klassen, which encourages the consumption of only raw foods in their "natural state, basically fruits, vegetables, grains and nuts,"[101] getting plenty of physical exercise as well as abstinence from alcohol, nicotine, caffeine, sugar, preservatives, insecticides, narcotics and other drugs whether prescription or non-prescription.[102] Salubrious Living is considered mandatory to "fully practice" Creativity and a lawsuit is currently in place against the Bureau of Prisons to get it recognized as a religious dietary preference[103] for incarcerated adherents of the religious movement. See also Animal sacrifice The earliest records of vegetarianism as a concept and practice amongst a significant number of people are from ancient India, especially among the Hindus[1] and Jains.[2] Later records indicate that small groups within the ancient Greek civilizations in southern Italy and Greece also adopted some dietary habits similar to vegetarianism.[3] In both instances, the diet was closely connected with the idea of nonviolence toward animals (called ahimsa in India), and was promoted by religious groups and philosophers.[4] Following the Christianization of the Roman Empire in late antiquity (4th–6th centuries), vegetarianism nearly disappeared from Europe.[5] Several orders of monks in medieval Europe restricted or banned the consumption of meat for ascetic reasons, but none of them abstained from the consumption of fish; these monks were not vegetarians, but some were pescetarians.[6] Vegetarianism was to reemerge somewhat in Europe during the Renaissance[7] and became a more widespread practice during the 19th and 20th centuries. The figures for the percentage of the Western world which is vegetarian varies between 0.5% and 4% per Mintel data in September 2006.[8][citation needed] Contents 1 Ancient 1.1 Indian subcontinent 1.1.1 Early Jainism and Buddhism 1.1.2 Hinduism 1.2 Mediterranean Basin 1.2.1 Ancient Greece 1.2.2 Judaism 1.3 East and Southeast Asia 1.3.1 China 1.3.2 Japan 1.4 Orthodox Christianity 1.5 Christian antiquity and Middle Ages 2 Early modern period 3 19th century 3.1 Great Britain 3.1.1 Class 3.1.2 Women 3.2 United States 3.3 Other countries 4 20th century 5 Current situation 6 Historians of vegetarianism 7 See also 8 Notes 9 Sources 10 Further reading Ancient Indian subcontinent Early Jainism and Buddhism Further information: History of Jainism and History of Buddhism Vegetarianism in ancient India India is a strange country. People do not kill any living creatures, do not keep pigs and fowl, and do not sell live cattle. —Faxian, 4th/5th century CE Chinese pilgrim to India[9] Jain and Buddhist sources show that the principle of nonviolence toward animals was an established rule in both religions as early as the 6th century BCE.[10] The Jain concept, which is particularly strict, may be even older. Parshva, the 23rd Jain leader (Tirthankara) whom modern historians consider to be a historical figure, lived in the 8th or 7th century BCE. He is said to have preached nonviolence no less radically than it was practiced in the Jain community in the times of Mahavira (6th century BCE).[11] Between 4th and 1st centuries BCE, or perhaps as late as 5th century CE, ancient Indian philosopher Valluvar wrote an exclusive chapter on veganism or moral vegetarianism in his work Tirukkural, emphasizing unambiguously on non-animal diet (Chapter 26), non-harming (Chapter 32), and non-killing (Chapter 33).[12][13] Not everyone who refused to participate in any killing or injuring of animals also abstained from the consumption of meat.[14] Hence the question of Buddhist vegetarianism in the earliest stages of that religion's development is controversial. There are two schools of thought. One says that the Buddha and his followers ate meat offered to them by hosts or alms-givers if they had no reason to suspect that the animal had been slaughtered specifically for their sake.[15] The other one says that the Buddha and his community of monks (sangha) were strict vegetarians and the habit of accepting alms of meat was only tolerated later on, after a decline of discipline.[16] The first opinion is supported by several passages in the Pali version of the Tripitaka, the opposite one by some Mahayana texts.[17] All those sources were put into writing several centuries after the death of the Buddha.[18] They may reflect the conflicting positions of different wings or currents within the Buddhist community in its early stage.[19] According to the Vinaya Pitaka, the first schism happened when the Buddha was still alive: a group of monks led by Devadatta left the community because they wanted stricter rules, including an unconditional ban on meat eating.[19] The Mahaparinibbana Sutta, which narrates the end of the Buddha's life, states that he died after eating sukara-maddava, a term translated by some as pork, by others as mushrooms (or an unknown vegetable).[20][21] The Buddhist emperor Ashoka (304–232 BCE) was a vegetarian,[22] and a determined promoter of nonviolence to animals. He promulgated detailed laws aimed at the protection of many species, abolished animal sacrifice at his court, and admonished the population to avoid all kinds of unnecessary killing and injury.[23] Ashoka has asserted protection to fauna, from his edicts: Beloved-of-the-Gods, King Piyadasi, has caused this Dhamma edict to be written.[1] Here (in my domain) no living beings are to be slaughtered or offered in sacrifice. Nor should festivals be held, for Beloved-of-the-Gods, King Piyadasi, sees much to object to in such festivals, although there are some festivals that Beloved-of-the-Gods, King Piyadasi, does approve of. Formerly, in the kitchen of Beloved-of-the-Gods, King Piyadasi, hundreds of thousands of animals were killed every day to make curry. But now with the writing of this Dhamma edict only three creatures, two peacocks and a deer are killed, and the deer not always. And in time, not even these three creatures will be killed.[24] —Edicts of Ashoka on 1st Major Rock Edict Twenty-six years after my coronation various animals were declared to be protected—parrots, mainas, aruna, ruddy geese, wild ducks, nandimukhas, gelatas, bats, queen ants, terrapins, boneless fish, vedareyaka, gangapuputaka, sankiya fish, tortoises, porcupines, squirrels, deer, bulls, okapinda, wild asses, wild pigeons, domestic pigeons and all four-footed creatures that are neither useful nor edible. Those nanny goats, ewes and sows which are with young or giving milk to their young are protected, and so are young ones less than six months old. Cocks are not to be caponized, husks hiding living beings are not to be burnt and forests are not to be burnt either without reason or to kill creatures. One animal is not to be fed to another. —Edicts of Ashoka on Fifth Pillar Theravada Buddhists used to observe the regulation of the Pali canon which allowed them to eat meat unless the animal had been slaughtered specifically for them.[25] In the Mahayana school some scriptures advocated vegetarianism; a particularly uncompromising one was the famous Lankavatara Sutra written in the fourth or fifth century CE.[26] Hinduism The 5th-century CE Tamil scholar Valluvar, in his Tirukkural, taught ahimsa and moral vegetarianism as personal virtues. The plaque in this statue of Valluvar at an animal sanctuary at Tiruvallur describes the Kural's teachings on ahimsa and non-killing, summing them up with the definition of veganism. Philosopher Michael Allen Fox asserts that "Hinduism has the most profound connection with a vegetarian way of life and the strongest claim to fostering and supporting it."[1] In the ancient Vedic period (between 1500 and 500 BCE), although the laws allowed the consumption of some kinds of meat, vegetarianism was encouraged.[27] Hinduism yields several foundations for vegetarianism as the Vedas, the oldest and sacred texts of Hinduism, assert that all creatures manifest the same life force and therefore merit equal care and compassion.[28] A number of Hindu texts place injunctions against meat eating and others like the Ramayana and Mahabharata advocate for a vegetarian diet.[29] In Hinduism, killing a cow is traditionally considered a sin.[30] Vegetarianism was, and still is, mandatory for Hindu yogis, both for the practitioners of Hatha Yoga[31] and for the disciples of the Vaishnava schools of Bhakti Yoga (especially the Gaudiya Vaishnavas). A bhakta (devotee) offers all his food to Vishnu or Krishna as prasad before eating it.[32] Only vegetarian food can be accepted as prasad.[33] According to Yogic thought, saattvik food (pure or having good impact on body) is meant to calm and purify the mind "enabling it to function at its maximum potential" and keep the body healthy. Saatvik foods consist of "cereals, fresh fruit, vegetables, legumes, nuts, sprouted seeds, whole grains and milk taken from a cow, which is allowed to have a natural birth, life and death including natural food, after satiating the needs of milk of its calf".[34] Shankar Narayan suggests that the origin of vegetarianism in India developed from the idea that balance needed to be restored. He claims, "Along with the development in civilisation, savagery also increased and those who were helpless and voiceless among both humans and non-human animals were more and more exploited and killed to satiate human needs and greed thus disturbing the balance of nature. But there were also many serious attempts to bring back the humanity to sanity and restore balance from time to time." He also says that the idea of living in harmony with nature became central to the rulers and kings.[34] Mediterranean Basin See also: Classical antiquity The Greek philosopher and religious teacher Pythagoras (570 BC – 495 BC) is said to have advocated vegetarianism, but it is more likely that he only prohibited his followers from consuming certain kinds of meat.[35] Later Pythagoreans did practice various forms of vegetarianism.[35][36] Ancient Greece In Ancient Greece during Classical antiquity, the vegetarian diet was called abstinence from beings with a soul (Ancient Greek: ἀποχὴ ἐμψύχων).[37] As a principle or deliberate way of life it was always limited temperature Primary Akt ρ Akt and glyceraldehyde phosphate dehydrogenase GAPDH antibodies were used in PBS with % defatted dry milk and % Tween at : and : respectively according to the manufacturer’s instructions After extensive washing secondary HRP linked antibodies were used at a : dilution Specific proteins were visualized using the enhanced chemoluminescent substrate Apoptosis Necrosis Assay Caco and MDA MB cells were incubated with a complete medium containing nanoparticles at mg mL for h Fluorescein isothiocyanate FITC conjugated annexin V and propidium iodide staining Annexin V FITC Assay Kit Cayman Chemical was used according to the manufacturer’s instructions and the fluorescence intensity emission was measured SpectraMax M e Molecular Devices microplate reader at and nm after excitation at and nm respectively Three independent experiments each in triplicate were performed Assessment of the Internalization Pathway Caco and MDA MB cells were pretreated with μm of chloroquine μg mL filipin μg mL amiloride and μm of monodansylcadaverine in minimal medium for min After treatment with the inhibitor the medium was replaced with a fresh one containing mg mL polymer for h Finally the cell viability assay was carried out as described above Three independent experiments each in triplicate were performed Assessment of Intracellular Trafficking Caco and MDA MB cells were preincubated with μm of Pepstatin A at °C in each cell culture in complete medium for h After Pepstatin A treatment the medium was replaced with a fresh one containing mg mL polymer for h The cell viability was then determined as described above Three independent experiments each in triplicate were performed Statistical Analysis Data are reported as mean ± standard deviation SD n = Statistical analysis involved a variance analysis in combination with a subsequent analysis using the Bonferroni method A p value of less than was considered to be statistically significant p < p < p < Data were handled using SPSS Statistics software version IBM Biomacromolecules Article DOI: acs biomac b Biomacromolecules − RESULTS AND DISCUSSION ELR Design ELRs are able to self assemble into different structures depending on their composition with amphiphilic ELR based diblocks forming vesicles or micelles above their transition temperature The genetic design of ELRs allows us to control their characteristic Tt below the physiological temperature of °C thus resulting in coacervate formation One disadvantage of therapeutic molecules such as drugs or peptides is their short circulating half lives thus meaning that frequent administration of high concentrations is required to obtain the therapeutic level Therapeutic agents have to pass through several biological barriers to reach the tumor tissue and ensure an effective dose Furthermore systemic administration results in high toxicity especially for healthy tissues ELR based carriers play an interesting role regarding overcoming all these limitations as they are able to extend the circulating half life of therapeutic peptides or drugs and also improve their targeting and pharmacokinetics We designed two different polymers control and Akt in both based on an amphiphilic backbone consisting of a hydrophilic block formed by glutamic acid and a hydrophobic block formed by isoleucine The glutamic acid block is based on the monomer VPGVG VPGEG VPGVG whereas the isoleucine block is based on VGIPG This amphiphilic ELR construct has been shown to self assemble into highly monodisperse and stable nanoparticles with a size of nm We also included a small sequence containing three lysine residues to which different molecules can be attached by covalent binding at the amino terminus According to the literature we expected that our polymers would enter the cell via endosomes and continue to lysosomes; therefore we included an LAEL sequence a small peptide that undergoes a structural change from random coil to a helix after acidification of the pH in the endosome lysosome pH and triggers destabilization and permeabilization of the vesicle membrane thereby allowing endosomal escape in both polymers The control ELR construct was designed with an amphiphilic backbone containing the three lysine residues and the LAEL sequence This construct was used to clarify any effect of the ELR modules Moreover the LAEL sequence was included in the control polymer to study possible cytotoxicity due to internalization of the nanoparticles and their escape from endosomes lysosomes Furthermore we added different bioactive blocks to the functional polymer For example cathepsin D CatD is a lysosomal aspartyl endopeptidase that is overexpressed in cancer cells compared to normal cells and is thought to promote tumor invasion and growth As such we included a cathepsin D sensitive sequence to allow its enzymatic action and the Akt inhibitor to reach the cytoplasm To allow the Akt inhibitor to escape from the endosome lysosome we added the H codifying sequence a histidine rich peptide that undergoes a conformational change from a β structure to a disordered structure at acidic pH as a result of protonation of the imidazole ring in histidine and provides lysosomal membrane permeation Finally we included the sequence codifying the peptide Akt in a small peptide of fifteen amino acids which acts as an inhibitor of protein kinase Akt Indeed this inhibitor prevents Ser phosphorylation which is a key step upon binding to Akt protein before being active in the cellular cytoplasm and playing a key role in multiple signaling pathways In light of the above our hypothesis for the mechanism of action is that once CatD has digested the cathepsin D sensitive sequence the H peptide and inhibitor will be released into the cellular cytoplasm and will be able to bind to Akt protein and block it The amino acid sequences for the


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christine-black christine-chavert christine-neona christine-rigoler christy-canyon cicciolina cindi-stephens cindy-carver cindy-crawford cindy-more cindy-shepard cindy-wong cinthya-marinho clair-dia claire-robbins claude-janna claudia-jackson claudia-jamsson claudia-mehringer claudia-nero claudia-van-statt claudia-zante claudine-beccarie clea-carson cleo-nichole cleo-patra cody-lane cody-love cody-nicole coffee-brown colleen-brennan connie-bennett connie-peterson constance-money copper-penny coreena corey-everson corinne-lemoine corneliah cory-everson cory-wolf courtney courtney-cummz courtney-james cris-cassidy crissy-moran cris-taliana crystal-breeze crystal-dawn crystal-holland crystal-knight crystal-lake crystal-lovin crystal-sync csilla-kalnay cuban-bee cynara-fox cyndee-summers cynthia-black cynthia-brooks cynthia-hammers cynthia-lavigne dagmar-lost daisy-layne dallas-miko dana-dylan dana-lynn danica-rhea daniela-nanou daniela-schiffer daniele-troeger daniella daniella-schiffer danielle danielle-foxxx danielle-rodgers danny-ricci danyel-cheeks daphne daphne-rosen darby-lloyd-rains darla-crane darla-delovely davia-ardell dayton-rain debbie-northrup debbie-revenge debbie-van-gils debi-diamond debi-jointed debra-lynn deidra-hopkins deidre-holland delania-raffino delia-moore delphine-thail delta-force delta-white demi-moor denice-klarskov denise-derringer denise-dior denise-sloan desiree-cousteau desiree-foxx desiree-lane desiree-west deva-station devin-devasquez devinn-lane devon-shire dia diana-holt diana-kisabonyi diana-siefert diana-stevenson diane-dubois diane-richards diane-sloan diane-suresne dido-angel dillan-lauren dina-deville dina-jewel dina-pearl ditty-blue diva divinity-love djiana dolly-darkley dominique dominique-dewitt dominique-saint-claire donna-hart donna-marie dorle-buchner dorothy-lemay dorothy-onan drea drimla dru-berrymore dusty-rose dyanna-lauren ebony-ayes edina-blond edita-ungerova edwige-faillel eileen-wells elaine-southern elena-berkova elena-maria-ricci eleonore-melzer elisabeth-bure elis-black elise elise-di-medici elle-devyne elle-rio elodie-delage elsa-maroussia elza-brown emili-doll emily-evermoore emily-george emily-jewel emmanuelle-pareze envy-mi erica-boyer erica-eaton erica-havens erica-idol erica-lauren erika-bella erika-cool erika-heaven erika-lockett esme-monroe eva-allen eva-angel eva-dionisio eva-gross eva-kleber eva-lux eva-uettori eve-laurence evelyne-lang evie-delatosso fabiana-venturi faith-stevens fallon fanny-garreau fanny-steel faye-runaway flame flick-shagwell flore-soller flower france-lomay france-quenie francoise frankie-leigh gabriella gabriella-mirelba gabriella-vincze gail-force gail-palmer gail-sterling georgette-saunders georgia-peach georgina-spelvin gia-givanna gianna-lynn gili-sky gina-carrera gina-gianetti gina-janssen gina-lee gina-martell gina-valentino ginger-jay ginger-lee ginger-lynn ginny-noack giovanna gisela-schwarz giselle-monet gladys-laroche gloria-leonard 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